The Comparative Enzymology and Cell Origin of Rat Hepatomas
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چکیده
Received for publication August 16, 1965. to parenchymal liver cells in both morphology (18, 19) and enzyme pattern (@1, @6). This hepa toma has been called a “minimal-deviation― tu mor in contradistinction to multiple-deviation tu mors which may differ from their normal pro genitor so much as to make the identification of the cell of origin difficult if not impossible (@5). In addition, the multiple-deviation tumors exhibit many biochemical properties that may be ir relevant to the process of carcinogenesis as Weber and Cantero have also@recognized (30). Further studies were undertaken to determine whether primary hepatornas of the minimal
منابع مشابه
The comparative enzymology and cell origin of rat hepatomas. IV. Pyrimidine metabolism in minimal-deviation tumors.
rived from the primary tumors induced with ethionine. Normal liver, Dunning L-C18 hepatoma, and Morris 5123 hepatoma were similar in having almost no deoxycytidylate deaminase, whereas the Novikoff hepatoma was shown to be a rich source of the enzyme, converting 400-600 ¿¿moles substrate/gm protein/hr. Other hepatomas had values between 50 and 500 /umoles/gm protein/hr. The Morris 5123 hepato...
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Enzyme assays for deoxycytidylate deaminase and thymine catabolism were carried out on ten rat hepatomas, including the Novikoff, Dunning L-C18, McCoy MDAB, and the Morris 3683, 3924A, and 5128 hepatomas, plus primary hepatomas produced by feeding ethionine or 8'-methyl-4-dimethylaminoazobenzene, and hepatomas derived from the primary tumors induced with ethionine. Normal liver, Dunning L-C18 h...
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It is now well established that the type la of serine/threonine protein phosphatases (PP1α) may be implicated in malignant phenotype. Although the PP1α mRNA level increased in livers at preneo-plastic stages of hepatocarinogenesis and all of examined rat ascites hepatomas, unexpectedly, dra-matically decreased in some of primary hepatomas. In order to elucidate the low level of PP1α mRNA in som...
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The activities of glucose-6-phosphatase, glutamate dehydrogcnase, and choline ox[dase were determined in some or all of ten rat hepatomas, including the Novikoff, Dunning L-C18, McCoy MDAB, and the Morris 3683, 39524A, and 51~3 hepatomas, together with primary hepatomas produced by feeding ethionine or 3%nethyl-4dimethylaminoazobenzene, and transplanted hepatomas derived from the primary tumors...
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Mitochondrial malate dehydrogenase [L-malate, nicotinamide adenine dinucleotide oxidoreductase (EC 1.1.1.37)] from Morris rat hepatomas grown in vivo and from Morris rat hepatoma 7288C grown in cell culture (hepatic tumor cells) has been maximally purified for comparison with mitochondrial malate dehydrogenase from normal rat liver. A rabbit antiserum directed against normal rat liver mitochond...
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